In situ survival of genetically engineered microorganisms in a tropical aquatic environment
نویسندگان
چکیده
منابع مشابه
Microcosm for assessing survival of genetically engineered microorganisms in aquatic environments.
Laboratory-contained microcosms are important for studying the fate and survival of genetically engineered microorganisms. In this study, we describe a simple aquatic microcosm that utilizes survival chambers in a flowthrough or static renewal system. The model was used to study the survival of genetically engineered and wild-type strains of Escherichia coli and Pseudomonas putida in the lake w...
متن کاملBiodegradation: Involved Microorganisms and Genetically Engineered Microorganisms
Biodegradation is defined as the biologically catalyzed reduction in complexity of chemical compounds [1]. Indeed, biodegradation is the process by which organic substances are broken down into smaller compounds by living microbial organisms [2]. When biodegradation is complete, the process is called "mineralization". However, in most cases the term biodegra‐ dation is generally used to describ...
متن کاملSurvival and function of a genetically engineered Pseudomonad in aquatic sediment microcosms.
Pseudomonas sp. strain B13 FR1(pFRC20P) is a genetically engineered microorganism (GEM) which is able to degrade chloro- and methylaromatics through a constructed ortho cleavage pathway. The fate of the GEM and its ability to degrade substituted aromatic compounds in two different aquatic sediments was investigated by using a microcosm system which consisted of intact layered sediment cores wit...
متن کاملUse of green fluorescent protein to monitor survival of genetically engineered bacteria in aquatic environments.
Many methods for detecting model genetically engineered microorganisms (GEMs) in experimental ecosystems rely on cultivation of introduced cells. In this study, survival of Escherichia coli was monitored with the green fluorescent protein (GFP) gene. This approach allowed enumeration of GEMs by both plating and microscopy. Use of the GFP-marked GEMs revealed that E. coli persisted in stream wat...
متن کاملInfluence of Matric Potential on Survival and Activity of Genetically Engineered Ralstonia eutropha H850Lr
Although the application of biodegradative genetically engineered micro organisms (GEMs) for bioremediation is very promising, the risks of their release should be assessed before their introduction into the environment. Lux-marked Ralstonia eutropha H850Lr (formerly Alcaligenes eutrophus H850Lr) was introduced into sterile and non-sterile soil microcosms at matric potentials ?2.11, ?30, ?750, ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Environmental Toxicology and Water Quality
سال: 1996
ISSN: 1053-4725,1098-2256
DOI: 10.1002/(sici)1098-2256(1996)11:1<21::aid-tox4>3.0.co;2-b